The Keratan Sulphates: Structural Investigations Using Nmr Spectroscopy
نویسنده
چکیده
منابع مشابه
There are two major types of skeletal keratan sulphates.
High-field 1H-n.m.r.-spectroscopic studies supported by chemical carbohydrate analyses show that skeletal keratan sulphates (KS-II) of bovine origin may be sub-classified into two groups. Keratan sulphate chains from articular and intervertebral-disc cartilage (KS-II-A) contain two structural features, namely alpha(1----3)-fucose and alpha(2----6)-linked N-acetyl-neuraminic acid residues, that ...
متن کاملHigh-field n.m.r. studies of keratan sulphates. 1H and 13C assignments of keratan sulphate from shark cartilage.
Keratan sulphate was extracted from a shark/whale cartilage preparation and examined by 400 MHz 1H- and 100 MHz 13C-n.m.r. spectroscopy. Assignment of the majority of the resonances was facilitated by two-dimensional 13C-1H correlation by using a modified COLOC procedure and a COSY-45 experiment. The spectra are consistent with an N-acetyl-lactosamine repeating unit that is predominantly sulpha...
متن کاملOn the polylactose nature of chondroitin and keratan sulphates.
The recognition that keratan and chondroitin sulphates are based on the same polylactose backbone simplifies the study of heteroduplex formation between them, suggests insights into structure-function relationships in tissues, and permits new definitions of the linkage regions.
متن کاملCharacterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing α(2-3)-linked sialic acid and α(1-3)-linked fucose
Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-β-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. "H-NMR spectroscopic analysis of the products revealed one containing both α(2-3)-linked sialic aci...
متن کاملConstant and variable domains of different disaccharide structure in corneal keratan sulphate chains.
Four peptidokeratan sulphate fractions of different Mr and degree of sulphation were cut from the pig corneal keratan sulphate distribution spectrum. After exhaustive digestion with keratanase, the fragments were separated on DEAE-Sephacel and Bio-Gel P-10 and analysed for their Mr, degree of sulphation and amino sugar and neutral sugar content. It was found that every glycosaminoglycan chain i...
متن کامل